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anti gys  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti gys
    Anti Gys, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glycogen+synthase/Phospho-Glycogen+Synthase+(Ser641)+XP+Rabbit+mAb/pm41830465-118-45-47
    Average 94 stars, based on 33 article reviews
    anti gys - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Protopanaxadiol stimulates glucose consumption by modulating the AMP-activated protein kinase pathway in myotubes, hepatoma cells, and adipocytes
    Article Snippet: .. The blots were incubated with primary antibodies against IRS-1, p-IRS-1, phosphoinositide 3-kinase (PI3K), p-PI3K, Akt, p-Akt, AMP-activated protein kinase α (AMPKα), p-AMPKα, glycogen synthase kinase-3β (GSK-3β), p-GSK-3β, glycogen synthase, p-glycogen synthase, glucose-6-phosphatase (G6Pase), phosphoenolpyruvate carboxykinase (PCK1/PEPC), glyceraldehyde 3-phosphate dehydrogenase, and secondary antibodies (Cell Signaling Technology) overnight at 4 °C. .. The blots were treated with ECL Plus Western blotting detection reagent (Amersham Biosciences, Piscataway, NJ, USA).

    Article Title: The proteoglycan decorin does not influence adiposity, glucose tolerance, or aerobic exercise capacity in mice.
    Article Snippet: For total protein measurements the membranes were either stained with Ponceau S, or visualized with TGX stain- free technology (Bio- Rad, Hercules, CA, USA) for fluorescent detection of proteins. .. The membranes were then blocked in Tris buffered saline containing 0.1% Tween- 20 and 5% BSA, and incubated over night with primary antibodies in 2.5% BSA in TBS- T. The following antibodies were used: Total OXPHOS rodent antibody cocktail (MitoScience LLC Cat# MS604, RRID:AB_2629281), glycogen synthase (Cell Signaling Technology Cat# 3886, RRID:AB_2116392), phospho- glycogen synthase (Cell Signaling Technology Cat# 3891, RRID:AB_2116390), Ppargc1a (Santa Cruz Biotechnology Cat# sc- 13067, RRID:AB_2166218), Tnf (Cell Signaling Technology Cat# 3707, RRID:AB_2240625), PI3 Kinase p85 (Millipore Cat# 06- 497, RRID:AB_310141), Stat- 3 (Cell Signaling Technology Cat# 4904, RRID:AB_331269), and phopho- Stat3 ((Cell Signaling Technology Cat# 9167, RRID:AB_561284)). .. For detection we used HRP- conjugated secondary antibodies (Jackson ImmunoResearch Labs Cat# 111- 035- 144/RRID:AB_2307391 and Cat# 115- 035- 146/ RRID:AB_2307392), ECL substrate (Cat# 34076, Thermo Scientific, Rockford, IL, USA) and the ChemiDocTM Touch Imaging System (Bio- Rad, Hercules, CA, USA).

    Saline:

    Article Title: The proteoglycan decorin does not influence adiposity, glucose tolerance, or aerobic exercise capacity in mice.
    Article Snippet: For total protein measurements the membranes were either stained with Ponceau S, or visualized with TGX stain- free technology (Bio- Rad, Hercules, CA, USA) for fluorescent detection of proteins. .. The membranes were then blocked in Tris buffered saline containing 0.1% Tween- 20 and 5% BSA, and incubated over night with primary antibodies in 2.5% BSA in TBS- T. The following antibodies were used: Total OXPHOS rodent antibody cocktail (MitoScience LLC Cat# MS604, RRID:AB_2629281), glycogen synthase (Cell Signaling Technology Cat# 3886, RRID:AB_2116392), phospho- glycogen synthase (Cell Signaling Technology Cat# 3891, RRID:AB_2116390), Ppargc1a (Santa Cruz Biotechnology Cat# sc- 13067, RRID:AB_2166218), Tnf (Cell Signaling Technology Cat# 3707, RRID:AB_2240625), PI3 Kinase p85 (Millipore Cat# 06- 497, RRID:AB_310141), Stat- 3 (Cell Signaling Technology Cat# 4904, RRID:AB_331269), and phopho- Stat3 ((Cell Signaling Technology Cat# 9167, RRID:AB_561284)). .. For detection we used HRP- conjugated secondary antibodies (Jackson ImmunoResearch Labs Cat# 111- 035- 144/RRID:AB_2307391 and Cat# 115- 035- 146/ RRID:AB_2307392), ECL substrate (Cat# 34076, Thermo Scientific, Rockford, IL, USA) and the ChemiDocTM Touch Imaging System (Bio- Rad, Hercules, CA, USA).

    other:

    Article Title: Mitochondrial Redox Status Regulates Glycogen Metabolism via Glycogen Phosphorylase Activity
    Article Snippet: Muscle samples were lysed in NP-40 lysis buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl; 1% NP-40; containing a protease inhibitor cocktail (Roche Diagnostics, Tokyo, Japan) and phosphatase inhibitors (Roche).



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    Image Search Results


    Neuroprotective effects of nervonic acid (NA) on multiple neuronal cell types (A). Immunofluorescence staining indicating the (B) neuronal nuclei (NeuN), (D) nestin, (F) arginase 1 (ARG1), (H) inducible nitric oxide synthase (iNOS), and (J) myelin basic protein (MBP) expression. Scalebar: 50 μm. (C, E, G, I, and K) The quantitative analysis of (B), (D), (F), (H), and (J), respectively. n = 3, **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05. Mean ± SD.

    Journal: Biomaterials Research

    Article Title: Nervonic Acid from Malania oleifera Reverses Parkinson’s Disease by Regulating Oxidative Stress, Neuroinflammation, and Gut Microbiota

    doi: 10.34133/bmr.0349

    Figure Lengend Snippet: Neuroprotective effects of nervonic acid (NA) on multiple neuronal cell types (A). Immunofluorescence staining indicating the (B) neuronal nuclei (NeuN), (D) nestin, (F) arginase 1 (ARG1), (H) inducible nitric oxide synthase (iNOS), and (J) myelin basic protein (MBP) expression. Scalebar: 50 μm. (C, E, G, I, and K) The quantitative analysis of (B), (D), (F), (H), and (J), respectively. n = 3, **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05. Mean ± SD.

    Article Snippet: Primary antibody incubations were performed overnight at 4 °C using the following dilutions: glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 1:10,000, 60004-1-Ig, Proteintech), sequestosome 1 (SQSTM1/p62; 1:2,000, ab56416, Abcam), Parkin (1:2,000, A11172, ABclonal), glycogen synthase kinase 3β (GSK-3β; 1:2,000, A11731, ABclonal), TNF-α (1:1,000, BA0131, BOSTER), and α-synuclein (1:1,000, ab212184, Abcam).

    Techniques: Immunofluorescence, Staining, Expressing

    Molecular mechanism of nervonic acid (NA) protects dopaminergic (DAergic) neurons. (A, E, and G) Western blot (WB) analysis and (B to D, F, and H) quantitative results of sequestosome 1 (SQSTM1/p62), Parkin, glycogen synthase kinase 3β (GSK-3β), tumor necrosis factor-α (TNF-α), and α-synuclein levels ( n = 3). (I) The schematic illustration of NA regulates PTEN-induced kinase 1 (PINK1)–Parkin-mediated mitophagy, relieves oxidative stress, and alleviates Parkinson’s disease (PD) neuropathology. **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05. Mean ± SD.

    Journal: Biomaterials Research

    Article Title: Nervonic Acid from Malania oleifera Reverses Parkinson’s Disease by Regulating Oxidative Stress, Neuroinflammation, and Gut Microbiota

    doi: 10.34133/bmr.0349

    Figure Lengend Snippet: Molecular mechanism of nervonic acid (NA) protects dopaminergic (DAergic) neurons. (A, E, and G) Western blot (WB) analysis and (B to D, F, and H) quantitative results of sequestosome 1 (SQSTM1/p62), Parkin, glycogen synthase kinase 3β (GSK-3β), tumor necrosis factor-α (TNF-α), and α-synuclein levels ( n = 3). (I) The schematic illustration of NA regulates PTEN-induced kinase 1 (PINK1)–Parkin-mediated mitophagy, relieves oxidative stress, and alleviates Parkinson’s disease (PD) neuropathology. **** P < 0.0001, *** P < 0.001, ** P < 0.01, and * P < 0.05. Mean ± SD.

    Article Snippet: Primary antibody incubations were performed overnight at 4 °C using the following dilutions: glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 1:10,000, 60004-1-Ig, Proteintech), sequestosome 1 (SQSTM1/p62; 1:2,000, ab56416, Abcam), Parkin (1:2,000, A11172, ABclonal), glycogen synthase kinase 3β (GSK-3β; 1:2,000, A11731, ABclonal), TNF-α (1:1,000, BA0131, BOSTER), and α-synuclein (1:1,000, ab212184, Abcam).

    Techniques: Western Blot

    The figures illustrate the docking interaction of paeoniflorin with the GSK3-Beta receptor (PDB ID: 1Q41). (A) Shows a surface representation of GSK3-β with paeoniflorin (yellow) bound in the active site, highlighted in white. (B) Presents a 2D interaction diagram, showing key hydrogen bonds and hydrophobic contacts between paeoniflorin and the receptor. (C) 3D model displays paeoniflorin (brown) docked within GSK3-β, highlighting important interactions. (D) Provides a close-up view of the binding site, focusing on hydrogen bonds and hydrophobic interactions, depicted within a ribbon diagram.

    Journal: Frontiers in Pharmacology

    Article Title: Enhanced therapeutic potential of paeoniflorin and vitamin B12 in intracerebropeduncle ethidium bromide-induced multiple sclerosis-like pathology

    doi: 10.3389/fphar.2026.1792674

    Figure Lengend Snippet: The figures illustrate the docking interaction of paeoniflorin with the GSK3-Beta receptor (PDB ID: 1Q41). (A) Shows a surface representation of GSK3-β with paeoniflorin (yellow) bound in the active site, highlighted in white. (B) Presents a 2D interaction diagram, showing key hydrogen bonds and hydrophobic contacts between paeoniflorin and the receptor. (C) 3D model displays paeoniflorin (brown) docked within GSK3-β, highlighting important interactions. (D) Provides a close-up view of the binding site, focusing on hydrogen bonds and hydrophobic interactions, depicted within a ribbon diagram.

    Article Snippet: The ELISA kits for evaluating cellular and molecular targets included GDNF [E-EL-H1495; Elabscience GFRA1 [PKSH033670; Elabscience]; RET [AN00810P; Elabscience], AKT [E-EL-R0807 98T, Elabscience, Wuhan, China] ( ); ERK1/2 [E-AB-70292; Elabscience] ( ); and GSK3-Beta [KLR0989, KRISHGEN, Maharashtra, India] ( ).

    Techniques: Binding Assay

    (A–H) PNN neuroprotective role in mitigating EBRO-induced alterations in levels of cellular and molecular targets in MS rat model: GDNF (A) , GFRA1 (B) , AKT (C) , ERK1/2 (D) , GSK3-Beta (E) , in brain homogenates, and GDNF, GFRA1, AKT, ERK1/2, GSK3-Beta in CSF levels (F–H) . Statistical analysis was performed using a one-way ANOVA followed by Tukey’s post hoc test to determine significant differences among groups (A–H) . Data were presented as mean ± standard deviation (SD), with statistical significance set at p < 0.01. Each experimental group consisted of eight wistar rats (n = 8). β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–H) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.

    Journal: Frontiers in Pharmacology

    Article Title: Enhanced therapeutic potential of paeoniflorin and vitamin B12 in intracerebropeduncle ethidium bromide-induced multiple sclerosis-like pathology

    doi: 10.3389/fphar.2026.1792674

    Figure Lengend Snippet: (A–H) PNN neuroprotective role in mitigating EBRO-induced alterations in levels of cellular and molecular targets in MS rat model: GDNF (A) , GFRA1 (B) , AKT (C) , ERK1/2 (D) , GSK3-Beta (E) , in brain homogenates, and GDNF, GFRA1, AKT, ERK1/2, GSK3-Beta in CSF levels (F–H) . Statistical analysis was performed using a one-way ANOVA followed by Tukey’s post hoc test to determine significant differences among groups (A–H) . Data were presented as mean ± standard deviation (SD), with statistical significance set at p < 0.01. Each experimental group consisted of eight wistar rats (n = 8). β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–H) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.

    Article Snippet: The ELISA kits for evaluating cellular and molecular targets included GDNF [E-EL-H1495; Elabscience GFRA1 [PKSH033670; Elabscience]; RET [AN00810P; Elabscience], AKT [E-EL-R0807 98T, Elabscience, Wuhan, China] ( ); ERK1/2 [E-AB-70292; Elabscience] ( ); and GSK3-Beta [KLR0989, KRISHGEN, Maharashtra, India] ( ).

    Techniques: Standard Deviation, Control